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Metabolic time courses with high sensitivity |
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Adam W. Barb, S. Khan Hekmatyar,
John N. Glushka and James H. Prestegard.
Journal of Magnetic Resonance
2011 (in press)
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Monitoring the chemical conversion of metabolites in cellular and living systems offers great potential for
diagnosing and understanding the etiology of disease. NMR can play an important role, but it has been limited
by its inherent low sensitivity. Hyperpolarization using dynamic nuclear polarization (DNP) provides a solution.
This paper illustrates improvements attainable by combining DNP and indirect detection. Detection of
15N in the side chain amide of glutamine (the most abundant free amino acid in the body) is shown
to be enhanced by a factor of 160,000 over direct 15N observation, and significant levels of enhancement
persist for more than a minute. |
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